Abstract
The dorsal raphe nucleus (DRN) is the origin of the central serotonin [5-hydroxytryptamine (5-HT)] system and plays an important role in the regulation of many physiological functions such as sleep/arousal, food intake and mood. In order to understand the regulatory mechanisms of 5-HT system, characterization of the types of neurons is necessary. We performed electrophysiological recordings in acute slices of glutamate decarboxylase 67-green fluorescent protein knock-in mice. We utilized this mouse to identify visually GABAergic cells. Especially, we examined postsynaptic responses mediated by 5-HT receptors between GABAergic and serotonergic cells in the DRN. Various current responses were elicited by 5-HT and 5-HT(1A) or 5-HT(2A/2C) receptor agonists in GABAergic cells. These results suggested that multiple 5-HT receptor subtypes overlap on GABAergic cells, and their combination might control 5-HT cells. Understanding the postsynaptic 5-HT feedback mechanisms may help to elucidate the 5-HT neurotransmitter system and develop novel therapeutic approaches.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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8-Hydroxy-2-(di-n-propylamino)tetralin / pharmacology
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Amphetamines / pharmacology
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Animals
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GABAergic Neurons / drug effects*
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GABAergic Neurons / metabolism
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Glutamate Decarboxylase / genetics
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Green Fluorescent Proteins / biosynthesis
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Green Fluorescent Proteins / genetics
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Immunohistochemistry
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Patch-Clamp Techniques
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Promoter Regions, Genetic
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Raphe Nuclei / cytology
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Raphe Nuclei / drug effects*
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Raphe Nuclei / metabolism
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Receptor, Serotonin, 5-HT1A / drug effects
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Receptor, Serotonin, 5-HT1A / metabolism
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Receptor, Serotonin, 5-HT2A / drug effects
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Receptor, Serotonin, 5-HT2A / metabolism
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Receptor, Serotonin, 5-HT2C / drug effects
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Receptor, Serotonin, 5-HT2C / metabolism
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Receptors, Serotonin / drug effects*
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Receptors, Serotonin / metabolism
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Serotonin / metabolism*
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Serotonin 5-HT1 Receptor Agonists / pharmacology*
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Serotonin 5-HT2 Receptor Agonists / pharmacology*
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Synaptic Potentials
Substances
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Amphetamines
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Receptor, Serotonin, 5-HT2A
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Receptor, Serotonin, 5-HT2C
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Receptors, Serotonin
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Serotonin 5-HT1 Receptor Agonists
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Serotonin 5-HT2 Receptor Agonists
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Receptor, Serotonin, 5-HT1A
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Green Fluorescent Proteins
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Serotonin
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8-Hydroxy-2-(di-n-propylamino)tetralin
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Glutamate Decarboxylase
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glutamate decarboxylase 1
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4-iodo-2,5-dimethoxyphenylisopropylamine