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Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia

Abstract

The modified adhesive removal (sticky-tape) test is an assessment of somatosensory dysfunction following cerebral ischemia in rats. This test is less time consuming than the original protocol by virtue of requiring minimal pre-training. We present a detailed protocol describing how to conduct the modified adhesive removal (sticky-tape) test. Following right middle cerebral artery occlusion (rMCAo) using an intraluminal filament, animals undergo the modified sticky-tape test (MST) on post-operative days 1, 3, 7 and 10. For the test, a non-removable tape sleeve is placed around the animal's paw and the time to remove the stimulus is measured. The time spent attending to this stimulus is also recorded. Animals undergoing MST for the first time demonstrate nearly-uniform excellent performance. However, following rMCAo, the ratio of left to right performance on the MST is significantly different at all time points. In short, the MST accurately assesses neurological dysfunction in rodents, not only with minimal pre-training, but also with accurate localization to the side of injury.

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Figure 1: Image depicting an animal undergoing the MST22.
Figure 2: MST performance expressed as a ratio of left/right.

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References

  1. Gladstone, D.J., Black, S.E. & Hakim, A.M. Heart & Stroke Foundation of Ontario Centre of Excellence in Stroke, Recovery Toward wisdom from failure: lessons from neuroprotective stroke trials and new therapeutic directions. Stroke 33, 2123–2136 (2002).

    Article  PubMed  Google Scholar 

  2. Fisher, M. Recommendations for standards regarding preclinical neuroprotective and restorative drug development. Stroke 30, 2752–2758 (1999).

    Article  Google Scholar 

  3. Tong, D. et al. Correlation of perfusion- and diffusion-weighted MRI with NIHSS score in acute (<6.5 h) ischemic stroke. Neurology 50, 864–870 (1998).

    Article  CAS  PubMed  Google Scholar 

  4. Corbett, D. & Nurse, S. The problem of assessing effective neuroprotection in experimental cerebral ischemia. Prog. Neurobiol. 54, 531–548 (1998).

    Article  CAS  PubMed  Google Scholar 

  5. Zhang, L., Chen, J., Li, Y., Zhang, Z.G. & Chopp, M. Quantitative measurement of motor and somatosensory impairments after mild (30 min) and severe (2 h) transient middle cerebral artery occlusion in rats. J. Neurol. Sci. 174, 141–146 (2000).

    Article  CAS  PubMed  Google Scholar 

  6. Hernandez, T.D. & Schallert, T. Seizures and recovery from experimental brain damage*1. Exp. Neurol. 102, 318–324 (1988).

    Article  CAS  PubMed  Google Scholar 

  7. Zhang, L. et al. A test for detecting long-term sensorimotor dysfunction in the mouse after focal cerebral ischemia. J. Neurosci. Methods 117, 207–214 (2002).

    Article  PubMed  Google Scholar 

  8. Stroemer, R.P., Kent, T.A., Hulsebosch, C.E. & Feeney, D.M. Enhanced neocortical neural sprouting, synaptogenesis, and behavioral recovery with D-amphetamine therapy after neocortical infarction in rats Editorial comment. Stroke 29, 2381–2395 (1998).

    Article  CAS  PubMed  Google Scholar 

  9. Aronowski, J., Samways, E., Strong, R., Rhoades, H.M. & Grotta, J.C. An alternative method for the quantitation of neuronal damage after experimental middle cerebral artery occlusion in rats: analysis of behavioral deficit. J. Cerebr. Blood Flow Metab. 16, 705–713 (1996).

    Article  CAS  Google Scholar 

  10. Schallert, T. & Whishaw, I.Q. Bilateral cutaneous stimulation of the somatosensory system in hemidecorticate rats. Behav. Neurosci. 98, 518–540 (1984).

    Article  CAS  PubMed  Google Scholar 

  11. Chen, J., Li, Y. & Chopp, M. Intracerebral transplantation of bone marrow with BDNF after MCAo in rat. Neuropharmacology 39, 711–716 (2000).

    Article  CAS  PubMed  Google Scholar 

  12. Zhang, R. et al. Activated neural stem cells contribute to stroke-induced neurogenesis and neuroblast migration toward the infarct boundary in adult rats. J. Cerebr. Blood Flow Metab. 24, 441–448 (2004).

    Article  Google Scholar 

  13. Li, Y., Chen, J. & Chopp, M. Cell proliferation and differentiation from ependymal, subependymal and choroid plexus cells in response to stroke in rats. J. Neurol. Sci. 193, 137–146 (2002).

    Article  PubMed  Google Scholar 

  14. Schabitz, W.R. et al. Effect of brain-derived neurotrophic factor treatment and forced arm use on functional motor recovery after small cortical ischemia. Stroke 35, 992–997 (2004).

    Article  PubMed  Google Scholar 

  15. Lu, D. et al. Intraarterial administration of marrow stromal cells in a rat model of traumatic brain injury. J. Neurotrauma 18, 813–819 (2001).

    Article  CAS  PubMed  Google Scholar 

  16. Li, Y. et al. Intracerebral transplantation of bone marrow stromal cells in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson's disease. Neurosci. Lett. 316, 67–70 (2001).

    Article  CAS  PubMed  Google Scholar 

  17. Markgraf, C.G. et al. Sensorimotor and cognitive consequences of middle cerebral artery occlusion in rats. Brain Res. 575, 238–246 (1992).

    Article  CAS  PubMed  Google Scholar 

  18. Andersen, A.B., Finger, S., Andersen, C.S. & Hoagland, N. Sensorimotor cortical lesion effects and treatment with nimodipine. Physiol. Behav. 47, 1045–1052 (1990).

    Article  CAS  PubMed  Google Scholar 

  19. Andersen, C.S., Andersen, A.B. & Finger, S. Neurological correlates of unilateral and bilateral 'strokes' of the middle cerebral artery in the rat. Physiol. Behav. 50, 263–269 (1991).

    Article  CAS  PubMed  Google Scholar 

  20. Markgraf, C.G. et al. Recovery of sensorimotor function after distal middle cerebral artery photothrombotic occlusion in rats. Stroke 25, 153–159 (1994).

    Article  CAS  PubMed  Google Scholar 

  21. Schallert, T. et al. Tactile extinction: distinguishing between sensorimotor and motor asymmetries in rats with unilateral nigrostriatal damage. Pharmacol. Biochem. Behav. 16, 455–462 (1982).

    Article  CAS  PubMed  Google Scholar 

  22. Sughrue, M.E. et al. An improved test of neurological dysfunction following transient focal cerebral ischemia in rats. J. Neurosci. Methods 151, 83–89 (2006).

    Article  PubMed  Google Scholar 

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Correspondence to Ricardo J Komotar.

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Komotar, R., Kim, G., Sughrue, M. et al. Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia. Nat Protoc 2, 2345–2347 (2007). https://doi.org/10.1038/nprot.2007.359

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