Microscopic resolution far beyond the diffraction limit is possible by localizing single molecules individually. This approach has now been demonstrated on living cells.
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Gustafsson, M. Super-resolution light microscopy goes live. Nat Methods 5, 385–387 (2008). https://doi.org/10.1038/nmeth0508-385
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DOI: https://doi.org/10.1038/nmeth0508-385
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