Abstract
The tail suspension test (TST) and forced swimming test (FST) are widely used for assessing antidepressant activity and depression-like behavior. We found that CS mice show negligible immobility in inescapable situations. Quantitative trait locus (QTL) mapping using CS and C57BL/6J mice revealed significant QTLs on chromosomes 4 (FST) and 5 (TST and FST). To identify the quantitative trait gene on chromosome 5, we narrowed the QTL interval to 0.5 Mb using several congenic and subcongenic strains. Ubiquitin-specific peptidase 46 (Usp46) with a lysine codon deletion was located in this region. This deletion affected nest building, muscimol-induced righting reflex and anti-immobility effects of imipramine. The muscimol-induced current in the hippocampal CA1 pyramidal neurons and hippocampal expression of the 67-kDa isoform of glutamic acid decarboxylase were significantly decreased in the Usp46 mutant mice compared to control mice. These phenotypes were rescued in transgenic mice with bacterial artificial chromosomes containing wild-type Usp46. Thus, Usp46 affects the immobility in the TST and FST, and it is implicated in the regulation of GABA action.
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Acknowledgements
We thank the late M. Ukai (Meijo University) for his helpful suggestions and for providing the facilities for the behavioral experiments, A. Yoshiki (RIKEN BRC) for his useful suggestions regarding the rescue experiment with transgenic mice, S. Yasuo (Johann Wolfgang Goethe-University Frankfurt) for her guidance on the in situ hybridization experiment, RIKEN BRC for providing the mice and the Nagoya University Radio-isotope Center for allowing us to use their facilities. This research was supported in part by a Grant-in-Aid for Scientific Research to S.E. and by the Academic Frontier Project for Private Universities (2007–2011) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to T.M and T.N.
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S.E. designed the experiments, supervised the project and prepared the manuscript. S.T. designed and performed most of the experiments, analyzed the data and prepared the manuscript. H.S. performed the behavioral tests. T.M. and T.K. supervised the behavioral experiments. T.N. organized GABA-related experiments, and M.Mi, T.A. and M.Ma. performed electrophysiological experiments. M.N. contributed to histological examination. J.K., S.I. and A.I. conducted the QTL analysis. K.A. supervised the production of BAC transgenic mice. Y.I. and S.I. contributed to congenic strain breeding and phenotyping. T.Y. provided the facilities and provided technical support.
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Tomida, S., Mamiya, T., Sakamaki, H. et al. Usp46 is a quantitative trait gene regulating mouse immobile behavior in the tail suspension and forced swimming tests. Nat Genet 41, 688–695 (2009). https://doi.org/10.1038/ng.344
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DOI: https://doi.org/10.1038/ng.344
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