Novelty preference predicts place preference conditioning to morphine and its oral consumption in rats
Introduction
Individual variability in susceptibility to drug addiction has been well established in human and rodents (Piazza et al., 1989, Volkow et al., 1999, DeWit et al., 2003). For some individuals, the first drug experience is perceived as aversive, whereas others rate it as a positive experience that could lead to recreational consumption. Moreover, although some individuals can maintain recreational use for long periods of time without any debilitating consequences, a proportion of individuals become rapidly addicted. It is therefore important to determine the factors that predispose to drug consumption.
In the three dimensions theory of personality proposed by Cloninger (1987), “the dimension of novelty seeking is hypothesized to be a heritable tendency towards exhilaration or excitement in response to novel stimuli or cues for potential rewards or potential relief of punishment, which leads to frequent exploratory activity in pursuit of potential rewards as well as active avoidance of monotony and potential punishment”. Zuckerman (1994) who includes the novelty seeking as a subscale in the sensation seeking personality super-trait, has defined it as the preference for new, complex and ambiguous stimuli. Numerous studies have shown the importance of this feature in predisposition to addiction.
Novelty seeking remains the best predictive index of drug use (Jaffe and Archer, 1987, Masse and Tremblay, 1997) probably because high sensation seekers would be more likely to experiment with recreational drugs. In men, novelty seeking also predicts the amount and frequency of alcohol consumption (Andrucci et al., 1989, Cherpitel, 1993) as well as the predisposition to relapse (Kravitz et al., 1999, Meszaros et al., 1999).
A number of rodent models have been devised to measure novelty-seeking behaviour (Dellu et al., 1996, Bardo et al., 1996). Novelty-induced place preference paradigm evaluates more specifically novelty-seeking behaviour in a non-stressful situation. In this model, animals having free-choice access to a novel or familiar environment spend more time in the novel one. In this situation, animals fail to display an increase in corticosterone levels suggesting that behaviours in this situation are not guided by stress (Misslin and Cigrang, 1986).
Only a few studies thus far have analysed the relationship between individual differences in novelty preference in free-choice situations and predisposition to drugs of abuse, mostly with amphetamine. In rats, novelty preference does not predict subsequent amphetamine-induced stimulation of locomotor activity (Robinet et al., 1998), but is positively correlated with oral consumption of amphetamine solution at a low concentration when measured in a two-bottle free-choice paradigm (Pelloux et al., 2004). By contrast, amphetamine self-administration is not influenced by individual differences in novelty preference (Klebaur et al., 2001). In the conditioned place preference paradigm, a procedure currently used to assess rewarding effects, high novelty seekers (HNS) show a greater amphetamine-induced CPP compared to low novelty seekers (LNS) (Robinet et al., 1998, Klebaur and Bardo, 1999). However, using fewer conditioning sessions, the relationship between preference for novelty and amphetamine-induced place conditioning is not apparent (Pelloux et al., 2004).
It appears important to determine whether the influence of novelty seeking on the vulnerability to the effects of psychostimulants could be extended to the effects of drugs belonging to distinct pharmacological classes. Indeed, the different drugs of abuse produce different subjective effects. For example, whereas amphetamine is a powerful mental and motor stimulant, opiates have relaxing and sedative effects. Recently, Zheng et al. (2003) found that the magnitude of morphine place conditioning was positively correlated with novelty-seeking behaviour in a free-choice situation, but not with activity in an inescapable novel environment. However, perseverance of exploration (i.e. delayed activity) in novel environments was shown to predict morphine place conditioning (Nadal et al., 2005). In the present study, we further analysed the relationship between free-choice novelty seeking and vulnerability to morphine. Rats were initially screened for their novelty preference in novelty test chambers. Then, voluntary oral consumption of morphine solutions was measured in a two-bottle free-choice procedure. In order to elucidate whether the bitter taste of morphine affected its intake, HNS and LNS were compared for their oral morphine consumption when the drug was diluted either in tap water or in a sweetened solution (5% sucrose). In addition, we studied whether high and low novelty seekers differed in oral consumption of quinine, a substance known for its bitter taste but that is devoid of abuse liability. Furthermore, the rewarding effect of morphine in high and low novelty seekers was assessed in the conditioned place preference paradigm (Carr et al., 1989, Bardo and Bevins, 2000).
Section snippets
Animals
Male outbred Wistar rats (180–200 g upon arrival, corresponding to 7 weeks old) were purchased from Charles River/IFFA CREDO (Saint Germain sur l'Arbresle, France). Four rats were housed in large Makrolon cages (L = 40 cm, W = 25 cm, H = 18 cm). They were maintained on a 12-h/12-h light/dark cycle (lights on at 07:00 a.m.), at a constant temperature (21 ± 1 °C), with laboratory chow and water ad libitum. The experiments were carried out between 09:00 a.m. and 7:00 p.m. They were conducted in accordance
Oral consumption of morphine in a free-choice paradigm
The time course of oral morphine consumption as a function of the concentration of the morphine solution is shown in Fig. 1. For both concentrations (25 and 50 mg/l), we observed a progressive decline in morphine intake during the 32-day free-choice period (F(7,705) = 57; P < 0.001). The morphine ratio defined as the consumption of morphine on the total fluid consumption also differed across time (F(7,705) = 55, P < 0.001). The main effect of concentration on morphine intake (F(1,705) = 13.4, P < 0.001)
Discussion
The results from the present study show that novelty preference is positively correlated with morphine consumption of a low, but not a high concentrated solution. Furthermore, our data indicate that high novelty preference is associated with a greater morphine-induced conditioned place preference at 5 mg/kg but not at 1.25 mg/kg.
Acknowledgement
This work was supported by grants from MILDT/INSERM (contract MILDT no. 4TX10H-A02075SP) and “Conseil Régional Haute Normandie”, France.
The authors would like to thank Dr. Kim Hellemans for assistance with the manuscript preparation.
References (29)
- et al.
Psychobiology of novelty seeking and drug seeking behavior
Behav Brain Res
(1996) The development of opiate addiction in the rat
Pharmacol Biochem Behav
(1996)- et al.
The role of mesoaccumbens-pallidal circuitry in novelty-induced behavioral activation
Neuroscience
(1995) - et al.
Individual differences in novelty seeking on the playground maze predict amphetamine conditioned place preference
Pharmacol Biochem Behav
(1999) - et al.
Does neophobia necessarily imply fear or anxiety?
Behav Processes
(1986) - et al.
Perseverance of exploration in novel environments predicts morphine place conditioning in rats
Behav Brain Res
(2005) - et al.
Individual differences in novelty-induced activity and the rewarding effects of novelty and amphetamine in rats
Behav Processes
(1998) - et al.
The neural basis of drug craving: an incentive-sensitization theory of addiction
Brain Res Rev
(1993) - et al.
Susceptibility to morphine place conditioning: relationship with stress-induced locomotion and novelty-seeking behavior in juvenile and adult rats
Pharmacol Biochem Behav
(2003) - et al.
The relationship of MMPI and Sensation Seeking Scales to adolescent drug use
J Pers Assess
(1989)
Conditioned place preference: what does it add to our preclinical understanding of drug reward?
Psychopharmacology
Conditioned place preference as a measure of drug reward
Alcohol, injury, and risk-taking behavior: data from a national sample
Alcohol Clin Exp Res.
A systematic method for clinical description and classification of personality variants. A proposal
Arch Gen Psychiatry
Cited by (29)
A novel method to study reward-context associations and drug-seeking behaviors
2020, Journal of Neuroscience MethodsCitation Excerpt :First, we compare our model with other animal models used in substance abuse research followed by a description of the advantages and limitations of our model. In research involving drugs of abuse, it is common to observe behavioral experiments that consist of consumption of drug-containing solutions in rodent home cages, in addition to conditioned place preference studies involving intraperitoneal injections (Pelloux et al., 2006; Thanos et al., 2005). In this paper we describe beneficial protocols that include oral self-administration of drug solutions in a specific context other than the home cage.
Environmental novelty modulates the induction and expression of single injection-induced behavioral sensitization to morphine
2018, Pharmacology Biochemistry and BehaviorCitation Excerpt :Using the place-preference paradigm, a positive correlation between the response to novelty and the magnitude of conditioning to Mor in rats has been observed (Nadal et al., 2005; Zheng et al., 2003). In addition, Pelloux et al. (2006) have shown that preference for novelty positively correlated with both Mor-induced place preference and Mor intake in rats. Thus, studies evaluating the individual response to the rewarding and reinforcing properties of Mor in rats facing new stimuli have demonstrated that novelty and Mor may activate similar neuronal substrates.
The Roots and Individual Diversity of Addiction
2017, On Human Nature: Biology, Psychology, Ethics, Politics, and ReligionExploration of a novel object in late adolescence predicts novelty-seeking behavior in adulthood: Associations among behavioral responses in four novelty-seeking tests
2016, Behavioural ProcessesCitation Excerpt :The locomotor activity exhibited in a novel environment from which there is no escape (e.g., horizontal activation in a circular corridor or vertical activation—rearing-in an open field) is frequently measured in sensation seeking tests (Piazza et al., 1989; Pawlak and Schwarting, 2002). By contrast, novelty seeking is commonly defined as a preference for a novel context (or object) compared with a familiar context (Pelloux et al., 2004, 2006, 2015; Pisula, 2003; Dellu et al., 1996), thus usually involving two-trial testing procedures (and, thus, learning/habituation processes) and giving animals a choice to either approach or avoid novelty (Bardo et al., 2013; Meyer et al., 2010). Finally, the hole-board (HB) test consists in an open field apparatus with equidistant holes in the floor in which head-dipping behavior is used as an index of novelty reactivity (File and Wardill, 1975).
- 1
Present address: Department of Experimental Psychology, University of Cambridge, Downing Street, Cambridge CB 2 3EB, United Kingdom.