Developmental neuroscienceQuantitative analysis of parvalbumin-immunoreactive cells in the human epileptic hippocampus
Section snippets
Experimental procedures
Human brain tissue was obtained from two sources: from autopsies (supplied by Dr. R. Alcaraz, Forensic Pathology Service, Basque Institute of Legal Medicine, Bilbao, Spain) and postoperative tissue from 11 patients, suffering pharmacoresistant TLE (Department of Neurosurgery, Hospital de la Princesa, Madrid, Spain).
According to the Helsinki Declaration, the patient’s consent was obtained in all cases (BMJ 1991;302:1194) and all protocols were approved by the institutional ethical committee
Results
The cytoarchitectonic division of the hippocampal fields (Fig. 1A) was established on basis of the descriptions in Amaral and Insausti (1990), but also considering the CA4 field according to the definition of Rosene and Van Hoesen (1987).
The distinction between CA1 and the subiculum was established by the presence of clusters of modified pyramidal cells in the superficial aspect of the subiculum (Fig. 1). The CA1 field was divided in three subfields to account for the regional differences in
Discussion
In the current study, we have used quantitative methods to show for the first time that the lower density of PV-ir cells is not directly related to the overall loss of neurons in the human sclerotic hippocampus. Indeed, this is the case both in the polymorphic layer of the DG and in the stratum pyramidale of the CA fields. We also observed a notable greater overall neuron density coupled with a severe decrease in the density of PV-ir cells in the subiculum of sclerotic hippocampi when compared
Acknowledgments
We thank Dr. R. G. Sola and his team (Neurosurgery Service, Hospital de la Princesa, Madrid, Spain) for providing the tissue from epileptic patients and for their contributions, and we thank R. F. Senso for technical assistance.
This work was supported by the following institutions: Spanish Ministry of Education and Science (BFI2003-02745, BFI2003-01018, BFU2006-13395); Comunidad de Madrid (grant 08.5/0027/2001.1); and research fellowships for A.A. (Ricerca Fondazione Mariani, Grant R-04-40) and
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2022, NeuroscienceCitation Excerpt :Malfunctioning of PV-containing basket cells as well as of SST-containing O-LM and bistratified cells has been associated with the development of epilepsy. Thus, TLE patients, even without signs of Ammon’s horn sclerosis, show a selective loss of PV-containing basket cells in the subiculum (Andrioli et al., 2007), and in animal models of TLE, PV-containing interneurons of the subiculum and of sector CA1 selectively degenerate (Dinocourt et al., 2003; Knopp et al., 2008; Drexel et al., 2011). Furthermore, silencing of PV-containing basket cells and axo-axonic cells of the subiculum results in recurrent series of pre- or interictal spikes (IS) and spontaneously recurrent seizures (Drexel et al., 2017).
- 1
Both authors contributed equally to this work.
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Present address: Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06520, USA.