Increased serotonin release in mice frontal cortex and hippocampus induced by acute physiological stressors

https://doi.org/10.1016/S0304-3940(02)00029-0Get rights and content

Abstract

The effects of acute physiological stressors (5 s tail pinch, handling and forced swimming at +25 and +5 °C for 3 min each) on serotonin (5-HT) release in the mouse brain were investigated using in vivo microdialysis. The extracellular 5-HT levels were determined by a newly developed highly-sensitive and selective high-performance liquid chromatography method based on derivatization with benzylamine and fluorescence detection. The basal levels of 5-HT in 3 min microdialysates from the ventral hippocampus and frontal cortex were 0.68±0.21 and 0.75±0.28 fmol/6 μl (n=24), respectively. All three stressors caused an immediate, significant and reversible increase (handling: 150%; swimming: 240%) of extracellular 5-HT levels in both brain structures, suggesting a more dynamic role played by the serotonergic system in response to acute stress.

Section snippets

Acknowledgements

This study was in part supported by the Advanced Materials Institute, Fukuoka University.

References (19)

There are more references available in the full text version of this article.

Cited by (86)

  • Ligand modulation of KCNQ-encoded (K<inf>V</inf>7) potassium channels in the heart and nervous system

    2021, European Journal of Pharmacology
    Citation Excerpt :

    In rodent models, acute stress can cause impaired retrieval of the hippocampus-dependent spatial memory (Li et al., 2008; Wong et al., 2007). Acute stress has been shown to raise the output of 5-hydoxytryptamine (5-HT) in the hippocampus (Amat et al., 1998; Fujino et al., 2002). 5-HT was reported as an inhibitor of M-current channels in mammalian neurons (Roepke et al., 2012).

View all citing articles on Scopus
View full text