Research paperRat hippocampal slices ‘in vitro’ display spontaneous epileptiform activity following long-term organotypic culture
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Imaging pathological activities of human brain tissue in organotypic culture
2018, Journal of Neuroscience MethodsOrganotypic Hippocampal Slice Cultures as a Model of Posttraumatic Epileptogenesis
2017, Models of Seizures and Epilepsy: Second EditionAn organotypic brain slice preparation from adult patients with temporal lobe epilepsy
2014, Journal of Neuroscience MethodsContribution of Aberrant GluK2-Containing Kainate Receptors to Chronic Seizures in Temporal Lobe Epilepsy
2014, Cell ReportsCitation Excerpt :Therefore, we demonstrate that aberrant GluK2-containing KARs at recurrent mossy fiber (rMF) synapses play a major role in chronic seizures in TLE. In an attempt to elucidate the role of KARs in spontaneous epileptiform discharges in the DG, we used organotypic hippocampal slice cultures treated with pilocarpine that display mossy fiber sprouting and spontaneous epileptiform activity (Figures 1A–1C and S1A) (Zimmer and Gähwiler, 1984; McBain et al., 1989; Gutiérrez and Heinemann, 1999; Thomas et al., 2005; Dyhrfjeld-Johnsen et al., 2010; Grabs et al., 1994; Albus et al., 2013). We observed a similar sprouting of rMFs in wild-type (WT) (n = 32) and GluK2−/− (n = 27) slices following incubation with pilocarpine as revealed by synaptoporin (SPO) staining (Grabs et al., 1994) (see Supplemental Experimental Procedures) (optical density [OD]: 26.1 ± 2.9 in WT and 25.0 ± 3.0 in GluK2−/−, p > 0.05, Figure 1A).
Network Control Mechanisms-Synaptogenesis and Epilepsy Development
2014, Neuronal Networks in Brain Function, CNS Disorders, and Therapeutics
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