Elsevier

Brain Research

Volume 447, Issue 2, 3 May 1988, Pages 369-375
Brain Research

Short communication
A new combination of WGA-HRP anterograde tracing and GABA immunocytochemistry applied to afferents of the cat inferior olive at the ultrastructural level

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Abstract

In order to identify cerebellar terminals in the cat inferior olive which contain γ-aminobutyric acid (GABA), a technique was developed combining anterograde transport of wheatgerm agglutinine-conjugated horseradish (WGA-HRP) with gold-immunocytochemistry. With this technique both the HRP reaction product and the immunogold labelling can be visualized in a single ultrathin section. Our results suggest that most, if not all the WGA-HRP-labelled cerebellar terminals in the rostal medial accessory olive (MAO) and the rostral principal olive (PO) are GABAergic. In an additional experiment the GABAergic innervation of the rostral MAO was studied in combination with WGA-HRP anterograde tracing from the rostral mesencephalon. In this case the WGA-HRP-labelled terminals were never found to be GABA-positive.

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This study was in part supported by a travel grant (R 92-105) rom the Netherlands Organization for the Advancement of Pure Research (Z.W.O.).

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The authors would like to thank Mrs. R.M.Y. Hartsteen for cutting nice ultrathin sections, Mr. R.C. Boer, Mr. F.H. Klink, Mr. J.v.d. Burg and Mr. H.G. Jansen for their technical assistance, Mr. E. Dalm for his assistance in surgery, Mrs. E. Klink for typing the manuscript, Mrs. P. van Alphen for her photographic assistance and Dr. N.M. Gerrits for reading the manuscript.

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