Persistent inflammation does not alter the number (Bmax) or affinity (Kd) of α4β2 nicotinic acetylcholine receptors in the rostral ventromedial medulla.
| Treatment | Bmax, fmol/mg protein | Kd, pm |
|---|---|---|
| 4 h | ||
| Saline | 22.5 ± 2.0 | 12.5 ± 2.4 |
| CFA | 23.6 ± 0.5 | 13.7 ± 0.7 |
| 4 d | ||
| Saline | 19.4 ± 2.1 | 12.5 ± 4.2 |
| CFA | 20.0 ± 1.4 | 12.3 ± 1.6 |
| 2 weeks | ||
| Saline | 20.0 ± 1.1 | 11.1 ± 2.0 |
| CFA | 18.9 ± 2.6 | 9.8 ± 3.6 |
Saturation isotherms were generated for the binding of [3H]epibatidine in membrane homogenates prepared from rats 4 h, 4 d, or 2 weeks after intraplantar injection of saline or CFA. Values represent the mean ± SEM. of three to five independent experiments. Two-way ANOVA (treatment × time) for each binding parameter identified no statistical differences.