1A | | See below | |
1B | Normal distribution | Two-way ANOVA (stress treatment × virus type) | Main effect of virus type: p = 0.0044 |
| | Main effect of stress treatment: p < 0.0001 | |
| | Sidak’s multiple-comparisons test | Interaction: p < 0.0001 |
| | Home cage–inescapable Stress | 95% CI: |
| | eYFP | 30.24–58.97 |
| | NpHR | −7.976 to 20.10 |
2B | Normal distribution | Two-way repeated-measures ANOVA | Main effect virus type: p = 0.0007 |
| | Sidak’s multiple comparisons test | Main effect time: p < 0.0001 |
| | eYFP – NpHR | Interaction: p = 0.0002 |
| | BL1 | 95% CI: |
| | BL2 | −43.11 to 65.40 |
| | BL3 | −59.67 to 48.84 |
| | BL4 | −47.73 to 60.78 |
| | S1 | −66.85 to 41.66 |
| | S2 | 33.82–142.3 |
| | S3 | −9.440 to 99.07 |
| | S4 | 7.267–115.8 |
| | S5 | 1.613–110.1 |
| | PS1 | 0.8602–109.4 |
| | PS2 | −5.678 to 102.8 |
| | PS3 | 13.43–121.9 |
| | PS4 | 2.628–111.1 |
| | Independent samples t test (AUC) | 0.5271–109.0 |
| | T, df | p = 0.0023 |
| | | t = 3.859 df = 12.00 |
2C | Normal distribution | Independent-samples t test for baseline JSI 5-HT values | Maine effect virus type: p = 0.0029 |
| | Two-way repeated-measures ANOVA | Main effect time: p < 0.0001 |
| | Sidak’s multiple-comparisons test | Interaction: p < 0.0001 |
| | eYFP – NpHR | 95% CI: |
| | BL1 | −31.60 to 60.99 |
| | BL2 | −32.35 to 60.23 |
| | BL3 | −67.83 to 24.76 |
| | BL4 | −41.90 to 50.69 |
| | JSI | 78.49–171.1 |
| | PI1 | −9.353 to 83.23 |
| | PI2 | −34.55 to 58.03 |
| | PI3 | −25.61 to 66.98 |
| | Independent-samples t test (AUC) | p = 0.0102 |
| | T, df | t = 3.580 df = 6.479 |
2D | Normal distribution | Independent-samples t test | p < 0.0001 |
| | T, df | t = 6.344 df = 9.716 |
2D | Normal distribution | Linear regression | p = 0.0125 |
| | | R2 = 0.3921 |
| | | R = −0.6257 |
| | T, df | t = 6.344 df = 9.716 |
3 | Normal distribution | Ordinary one-way ANOVA | p = 0.0065 |
| | Tukey’s post hoc test | 95% CI |
| | MHb Fos 0 h | 0.03556 to 2.589 |
| | HC vs ES | 0.4731 to 3.027 |
| | HC vs IS | -0.8394 to 1.714 |
| | ES vs IS | p < 0.0001 |
| | LHbM Fos 0 h | −37.59 to −19.54 |
| | HC vs ES | −34.15 to −16.10 |
| | HC vs IS | −5.587 to 12.46 |
| | ES vs IS | p = 0.1416 |
| | LHbL Fos 0 h | p = 0.3300 |
| | MHb Fos 2 h | p = 0.0013 |
| | LHbM Fos 2 h | 95% CI: |
| | HC vs ES | −38.21 to −8.789 |
| | HC vs IS | −34.40 to −4.977 |
| | ES vs IS | −10.90 to 18.52 |
| | LHbL Fos 2 h | p = 0.1888 |
| | MHb Fos 4 h | p = 0.8780 |
| | LHbM Fos 4 h | p = 0.8652 |
| | LHbL Fos 4 h | p = 0.0348 |
| | HC vs ES | 95% CI: |
| | HC vs IS | −3.706 to 0.4562 |
| | ES vs IS | −4.331 to −0.1688 |
| | | −2.706 to 1.456 |
4 | Normal distribution | Ordinary one-way ANOVA | 95% CI: |
| | Tukey’s post hoc test | p = 0.6651 |
| | Total FG MHb | p = 0.7163 |
| | Total FG LHbM | p = 0.5220 |
| | Total FG LHbL | p = 0.9074 |
| | Total Fos-IR MHb | p < 0.0001 |
| | Total Fos-IR LHbM | 95% CI: |
| | HC vs ES | −16.94 to −6.726 |
| | HC vs IS | −18.73 to −8.517 |
| | ES vs IS | −6.899 to 3.316 |
| | Total Fos-IR LHbL | p = 0.0109 |
| | HC vs ES | 95% CI: |
| | HC vs IS | −8.395 to −1.022 |
| | ES vs IS | −7.020 to 0.3534 |
| | Total % double-label MHb | −2.312 to 5.062 |
| | Total % double-label LHbM | p = 0.4152 |
| | HC vs ES | p < 0.0001 |
| | HC vs IS | 95% CI: |
| | ES vs IS | −21.49 to −7.661 |
| | Total % double-label LHbL | −18.44 to −4.614 |
| | | −3.866 to 9.960 |
| | | p = 0.0764 |