Statistics
| Figure | Data Structure | Type of test | p Values |
|---|---|---|---|
| 1 (inset) | Normal distribution | One-way ANOVA | 0.98 |
| 1a | Normal distribution | Two-way repeated-measures ANOVA (time × diet) Tukey’s post hoc test Day 1 Regular vs control Regular vs HFD Control vs HFD Day 2 Regular vs control Regular vs HFD Control vs HFD Day 3 Regular vs control Regular vs HFD Control vs HFD | <0.0001 95% CI −2.022 to 0.077 −1.518 to 0.581 −0.546 to 1.553 −1.887 to 0.211 −2.513 to −0.414 −1.675 to 0.424 −2.632 to −0.533 −3.762 to −1.663 |
| 1b | Normal distribution | One-way ANOVA | 0.31 |
| 1c | Normal distribution | One-way ANOVA | 0.93 |
| 1d | Normal distribution | One-way ANOVA Tukey’s post hoc test Regular vs control Regular vs HFD Control vs HFD | <0.0001 95% CI −5.787 to −0.827 −8.047 to −3.086 −4.740 to −0.221 |
| 2 | Normal distribution | One-way ANOVA Fisher’s LSD post hoc test Regular vs control Regular vs HFD Control vs HFD | 0.028 95% CI −0.378 to 0.180 −0.658 to −0.100 −0.559 to −0.001 |
| 3a | Normal distribution | One-way ANOVA | 0.194 |
| 3b | Normal distribution | One-way ANOVA | 0.131 |
| 3c | Normal distribution | One-way ANOVA Fisher’s LSD post hoc test Regular vs control Regular vs HFD Control vs HFD | 0.026 95% CI −29.17 to 9.432 −46.26 to −7.658 −35.74 to 1.556 |
| 3d | Normal distribution | One-way ANOVA Fisher’s LSD post hoc test Regular vs control Regular vs HFD Control vs HFD | 0.049 95% CI −16.12 to 16.28 −34.89 to −1.426 −34.44 to −2.038 |
| 3e | Normal distribution | One-way ANOVA Fisher’s LSD post hoc test Regular vs control Regular vs HFD Control vs HFD | 0.026 95% CI −55.03 to 47.63 −104.2 to −1.550 −98.77 to 0.406 |
| 3f | Normal distribution | One-way ANOVA Fisher’s LSD post hoc test Regular vs control Regular vs HFD Control vs HFD | 0.017 95% CI −21.42 to 15.11 −45.09 to −7.351 −41.33 to −4.796 |
| 4a | Normal distribution | Two-way ANOVA Diet Challenge Diet × challenge Scheffé’s post hoc test Regular:saline vs regular:LPS Regular:saline vs HFD:saline Regular:LPS vs HFD:LPS HFD:saline vs HFD:LPS | 0.002 <0.0001 0.040 0.008 0.071 0.010 0.0003 |
| 4b | Normal distribution | Two-way ANOVA diet challenge diet × challenge Scheffé’s post hoc test Regular:saline vs regular:LPS Regular:saline vs HFD:saline Regular:LPS vs HFD:LPS HFD:saline vs HFD:LPS | 0.040 <0.0001 0.031 0.0014 0.778 0.042 0.0007 |
| 4c | Normal distribution | Two-way ANOVA diet challenge diet × challenge Scheffé’s post hoc test Regular:saline vs regular:LPS Regular:saline vs HFD:saline Regular:LPS vs HFD:LPS HFD:saline vs HFD:LPS | 0.003 <0.0001 0.015 0.0003 0.091 0.013 0.0008 |
| 5a | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge Scheffé’s post hoc test Vehicle:saline:regular vs vehicle:saline:HFD Vehicle:LPS:regular vs vehicle:LPS:HFD Mife:saline:regular vs Mife:saline:HFD Mife:LPS:regular vs Mife:LPS:HFD Regular:saline:vehicle vs regular:saline:Mife Regular:LPS:vehicle vs regular:LPS:Mife HFD:saline:vehicle vs HFD:saline:Mife HFD:LPS:vehicle vs HFD:LPS:Mife Regular:vehicle:saline vs regular:vehicle:LPS Regular:Mife:saline vs regular:Mife:LPS HFD:vehicle:saline vs HFD:vehicle:LPS HFD:Mife:saline vs HFD:Mife:LPS | 0.0002 0.0053 <0.0001 0.105 0.038 <0.0001 0.033 0.083 0.0005 0.235 0.531 0.100 0.152 0.126 0.003 <0.0001 0.346 <0.0001 0.323 |
| 5b | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge Scheffé’s post hoc test Vehicle:saline:regular vs vehicle:saline:HFD Vehicle:LPS:regular vs vehicle:LPS:HFD Mife:saline:regular vs Mife:saline:HFD Mife:LPS:regular vs Mife:LPS:HFD Regular:saline:vehicle vs regular:saline:Mife Regular:LPS:vehicle vs regular:LPS:Mife HFD:saline:vehicle vs HFD:saline:Mife HFD:LPS:vehicle vs HFD:LPS:Mife Regular:vehicle:saline vs regular:vehicle:LPS Regular:Mife:saline vs regular:Mife:LPS HFD:vehicle:saline vs HFD:vehicle:LPS HFD:Mife:saline vs HFD:Mife:LPS | 0.572 0.543 <0.0001 0.002 0.211 0.171 0.003 0.173 0.011 0.364 0.133 0.088 0.115 0.500 0.014 <0.0001 0.003 <0.0001 0.004 |
| 5c | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge Scheffé’s post hoc test Vehicle:saline:regular vs vehicle:saline:HFD Vehicle:LPS:regular vs vehicle:LPS:HFD Mife:saline:regular vs Mife:saline:HFD Mife:LPS:regular vs Mife:LPS:HFD Regular:saline:vehicle vs regular:saline:Mife Regular:LPS:vehicle vs regular:LPS:Mife HFD:saline:vehicle vs HFD:saline:Mife HFD:LPS:vehicle vs HFD:LPS:Mife Regular:vehicle:saline vs regular:vehicle:LPS Regular:Mife:saline vs regular:Mife:LPS HFD:vehicle:saline vs HFD:vehicle:LPS HFD:Mife:saline vs HFD:Mife:LPS | 0.015 0.426 <0.0001 0.0004 0.005 0.237 0.0014 0.946 0.001 0.348 0.589 0.026 0.119 0.078 0.011 0.0005 0.032 <0.0001 0.031 |
| 5d | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge Scheffé’s post hoc test Vehicle:saline:regular vs vehicle:saline:HFD Vehicle:LPS:regular vs vehicle:LPS:HFD Mife:saline:regular vs Mife:saline:HFD Mife:LPS:regular vs Mife:LPS:HFD Regular:saline:vehicle vs regular:saline:Mife Regular:LPS:vehicle vs regular:LPS:Mife HFD:saline:vehicle vs HFD:saline:Mife HFD:LPS:vehicle vs HFD:LPS:Mife Regular:vehicle:saline vs regular:vehicle:LPS Regular:Mife:saline vs regular:Mife:LPS HFD:vehicle:saline vs HFD:vehicle:LPS HFD:Mife:saline vs HFD:Mife:LPS | 0.145 0.043 <0.0001 0.003 0.361 0.173 0.289 0.027 0.063 0.049 0.435 0.075 0.751 0.017 0.027 0.001 0.006 0.005 0.002 |
| 5e | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge Scheffé’s post hoc test Vehicle:saline:regular vs vehicle:saline:HFD Vehicle:LPS:regular vs vehicle:LPS:HFD Mife:saline:regular vs Mife:saline:HFD Mife:LPS:regular vs Mife:LPS:HFD Regular:saline:vehicle vs regular:saline:Mife Regular:LPS:vehicle vs regular:LPS:Mife HFD:saline:vehicle vs HFD:saline:Mife HFD:LPS:vehicle vs HFD:LPS:Mife Regular:vehicle:saline vs regular:vehicle:LPS Regular:Mife:saline vs regular:Mife:LPS HFD:vehicle:saline vs HFD:vehicle:LPS HFD:Mife:saline vs HFD:Mife:LPS | 0.009 0.226 0.01 0.006 0.942 0.044 0.060 0.010 0.027 0.130 0.037 0.145 0.072 0.939 0.021 0.010 0.017 0.091 0.75 |
| 5f | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge Scheffé’s post hoc test Vehicle:saline:regular vs vehicle:saline:HFD Vehicle:LPS:regular vs vehicle:LPS:HFD Mife:saline:regular vs Mife:saline:HFD Mife:LPS:regular vs Mife:LPS:HFD Regular:saline:vehicle vs regular:saline:Mife Regular:LPS:vehicle vs regular:LPS:Mife HFD:saline:vehicle vs HFD:saline:Mife HFD:LPS:vehicle vs HFD:LPS:Mife Regular:vehicle:saline vs regular:vehicle:LPS Regular:Mife:saline vs regular:Mife:LPS HFD:vehicle:saline vs HFD:vehicle:LPS HFD:Mife:saline vs HFD:Mife:LPS | 0.017 0.083 0.061 0.0007 0.269 0.111 0.459 <0.0001 0.136 0.760 0.149 0.074 0.375 0.049 0.0007 0.0001 0.876 0.338 0.535 |
| 6a | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge Scheffé’s post hoc test Vehicle:saline:regular vs vehicle:saline:HFD Vehicle:LPS:regular vs vehicle:LPS:HFD Mife:saline:regular vs Mife:saline:HFD Mife:LPS:regular vs Mife:LPS:HFD Regular:saline:vehicle vs regular:saline:Mife Regular:LPS:vehicle vs regular:LPS:Mife HFD:saline:vehicle vs HFD:saline:Mife HFD:LPS:vehicle vs HFD:LPS:Mife Regular:vehicle:saline vs regular:vehicle:LPS Regular:Mife:saline vs regular:Mife:LPS HFD:vehicle:saline vs HFD:vehicle:LPS HFD:Mife:saline vs HFD:Mife:LPS | 0.908 0.377 0.197 0.177 0.126 0.342 0.035 0.234 0.012 0.772 0.440 0.735 0.456 0.631 0.014 0.476 0.688 0.002 0.904 |
| 6b | Normal distribution | Three-way ANOVA Diet Treatment Challenge Diet × treatment Diet × challenge Treatment × challenge Diet × treatment × challenge | 0.154 0.722 0.297 0.157 0.518 0.740 0.051 |
Mife, Mifepristone.