Cannula placements for all intracranial microinjections of muscimol or saline: black dots represent contralateral cannula placements; white dots represent ipsilateral placements. The numbers identify the different subjects and their two cannulae.
A–D, Effects of contralateral or ipsilateral injections of saline or muscimol in POM and VTA on the latency to approach the female (A), the strutting frequency (B), the frequencies of mount attempts (C), and cloacal contact movements (D). Data are reported as the mean ± SEM. **p < 0.01, n.s. = p > 0.05 by Bonferroni’s post hoc tests following a significant interaction in the two-way ANOVA.
A, B, Photomicrographs showing sagittal sections in the quantification area (boxed area) in the POM ventral to anterior commissure (ac) in a male of the SB group (A) or of the control (B) group. C, Higher-magnification photomicrograph in POM illustrating cells that are Fos immunoreactive (blue arrow), BDA positive (black arrow), or double labeled for BDA and Fos (green arrow). D, Example of an injection site of BDA within VTA dorsal to the oculomotor nerve (3n).
A, B, Mean numbers of cells retrogradely labeled by BDA injected into the VTA (A) of Fos-ir cells (B) and the percentage of the BDA-positive cells that were simultaneously Fos immunoreactive in the POM, PVN, and LHA of the two groups of males that had engaged in sexual behavior (black bars) or had been kept as controls (white bars). Data are reported as the mean ± SEM. *p < 0.05.