Figure 2. Effects of 5d-RFSS on depressive-like behavior in C57BL/6J mice. A, Immobility time of 5d-RFSS C57BL/6J mice during the induction phase (days 1–5) forced-swim tests (FST; main effect of time, F(4,36) = 8.68, p = 0.00005; post hoc test, §p = 0.006, §§p = 0.001, §§§p = 0.0002 vs day 1), and of 5d-RFSS and nonstressed control mice during the test phase (day 37; t(1,18) = 3.44, *p = 0.003 vs nonstressed mice). B, Two-bottle SPTs before 5d-RFSS (SPT1) and after 5d-RFSS (SPT2 to SPT4). C, TST immobility time before 5d-RFSS (TST1) and after 5d-RFSS (TST2; main effect of time, F(1,18) = 9.14, p = 0.007; post hoc test, §p = 0.007 vs TST1). D–F, Distance traveled (t(1,18) = 0.43, p = 0.67; D), number of center zone entries (t(1,18) = 1.01, p = 0.33; E), and number of feces produced during the OFT (t(1,18) = 0.63, p = 0.53; F). G, Change in body weight (%) from day −3 to 36 (main effect of time, F(6,108) = 90.48, p < 0.00001; post hoc test, §p = 0.02, §§p = 0.0001 vs day −3). H, I, Food intake during induction phase (days 1–5; t(1,17) = 0.77, p = 0.45; H) and during days 8–36 (t(1,18) = 0.71, p = 0.49; I). NS, Not significant. n = 10/group for all experiments.