Figure 1. TBI activates mTORC1 signaling in the hippocampus. Mice received a moderate CCI at the age of 9 weeks and were sacrificed at 4, 24, 48, and 72 h and 1 week after injury as well as after sham injury (n = 3 for each group). A, Immunoblotting with antibodies against pS6, S6, and β-actin shows mTORC1 signaling activation in the hippocampus. B, Quantification of blots shown in A (*p < 0.05, **p < 0.01). C–N, Immunostaining with antibody against pS6 (red) shows mTORC1 signaling activation in the HDG after sham surgery (C) and 4 h (E), 24 h (G), 48 h (I), 72 h (K), and 1 week (M) after CCI, and in the SGZ at corresponding time points (D, F, H, J, L, N), respectively. DAPI staining shows the structure of HDG.